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Image Search Results
Journal: bioRxiv
Article Title: Inhibition of polo-like kinase 1 (PLK1) facilitates reactivation of gamma-herpesviruses in B-cell lymphomas and their elimination
doi: 10.1101/2020.10.08.330548
Figure Lengend Snippet: (A) Total RNAs were extracted from the fixed diffuse large B-cell lymphoma (DLBCL) tissue samples of HIV-negative (HIV-, n=3) and HIV-positive (HIV+, n=5) individuals. mRNA level of PLK1 in these tissue samples was measured by RT-PCR and normalized to GAPDH. (B) A PE-conjugated CD19 antibody was used for B cell enrichment from PBMCs of HIV-infected, aviremic patients (HIV+, n=5) or healthy donors (HIV-, n=5). Purity of isolated CD19 + B cells was confirmed by flow cytometry analysis. (C) mRNA level of PLK1 in above isolated B cells (B) was measured by RT-PCR and normalized to GAPDH. (D) Protein level of intracellular PLK1 in above isolated B cells (B) was measured by immunofluorescence. Percentage of PLK1-expressing cells was determined by flow cytometry analysis. (F) HIV-1 IIIB infected Jurkat cells were co-cultured with BJAB cells that were pre-stained with CFSE. Protein level of intracellular PLK1 in CFSE-labeled BJAB cells was measured by immunofluorescence. Percentage of PLK1-expressing cells was determined by flow cytometry analysis. (G-I) Protein level of PLK1 in BJAB (G), Atata-BX1 (H), or TREx BCBL1-Rta (I) cells treated with recombinant Nef (rNef) protein at the increasing dose or DMSO was measured by immunoblotting. GAPDH was used as a loading control. Results were calculated from n=3 independent experiments and presented as mean ± SD (* p<0.05; two-tailed paired Student t-test).
Article Snippet: Portion of isolated B cells was incubated with
Techniques: Reverse Transcription Polymerase Chain Reaction, Infection, Isolation, Flow Cytometry, Immunofluorescence, Expressing, Cell Culture, Staining, Labeling, Recombinant, Western Blot, Control, Two Tailed Test